Publication Type Journal Article
Title Prevalence and diversity of lactic acid bacteria in Chinese traditional sourdough revealed by culture dependent and pyrosequencing approaches
Authors Tongjie Liu Yun Li Jiancai Chen Faizan A. Sadiq Guohua Zhang Yang Li Guoqing He Christine Spence Michael Powell Taylor Jackson
Groups G1
Journal LWT-FOOD SCIENCE AND TECHNOLOGY
Year 2016
Month May
Volume 68
Number
Pages 91-97
Abstract The aim of this study was to investigate the microbiota of lactic acid bacteria (LAB) in fifteen different samples of Chinese traditional sourdough collected from different regions through culture dependent and pyrosequencing approaches. The median value of cell density of lactic acid bacteria was found to be 9.60 log cfu/g and the median values of pH and total titratable acidity were 3.69 and 11.77 ml, respectively. A total of 246 presumptive LAB strains were isolated and subjected to a clustering analysis based on (GTG)(5)-PCR fingerprinting profiles. Eleven species were found with an overwhelming predominance of Lactobacillus sanfranciscensis by culture dependent method. On the other hand, pyrosequencing revealed that more than 90\% of the bacteria belong to LAB in thirteen sourdough samples out of the fifteen. A total of twenty-four species were found by pyrosequencing and seventeen of them belonged to lactobacilli. Pyrosequencing indicated that Lactobacillus sanfranciscensis was the only species found existing among all the fifteen sourdoughs and that other species most frequently encountered in the samples included Weissella confusa, Lactobacillus plantarum and an unclassified Lactobacillus species. We conclude that LAB dominate in Chinese traditional sourdough and Lactobacillus sanfranciscensis is the predominant species in this specific niche. (C) 2015 Elsevier Ltd. All rights reserved.
DOI http://dx.doi.org/10.1016/j.lwt.2015.12.025
ISBN
Publisher
Book Title
ISSN 0023-6438
EISSN 1096-1127
Conference Name
Bibtex ID ISI:000370769700013
Observations
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